Rapid Counting Method of Microbes by Fluorescent Antibody Reaction

Visualization of Horse Sickness virus by the Fluorescent antibody technique

Rapid fluorescein and protein assay method for fluorescent-antibody conjugates.

A method is presented for the rapid determination of the fluorescein content, protein content, and fluorescein-to-protein ratio for immune globulin conjugates with fluorescein isothiocyanate as the fluor. This method is based on the absorbance of the fluorescent antibody at those wavelengths primarily associated with the fluorescein and gamma-globulin fractions, and permits these materials to b...

Rapid fluorescent-antibody staining technique.

The rapid fluorescent-antibody staining technique described by Kellogg and Deacon for staining Neisseria gonorrhoeae and Treponema pallidum was applied to fluorescent-antibody tests for group A streptococci and enteropathogenic Escherichia coli. Results obtained with this staining technique were compared with results using the conventional staining procedure; excellent correlation was obtained....

Rapid fluorescent-antibody conjugation procedure.

This note describes a technique developed for rapid preparation of specific and brilliant conjugates for direct staining of rickettsial agents in yolk-sac, tick, and vertebrate tissues.

Rapid Method for Counting Reticulocytes.

Among the several labour-saving methods for counting reticulocytes reviewed by Dacie and Lewis (1963) the least laborious is the Miller count described by Brecher and Schneiderman (1950). For this method the Miller graticule, a microscope eyepiece graticule with a square of side 7 mm. with a square of one ninth the area in one corner, is used and consecutive fields are counted, reticulocytes in...